色谱

色谱 ›› 2010, Vol. 28 ›› Issue (11): 1015-1019.DOI: 10.3724/SP.J.1123.2010.01015

• 研究论文 • 上一篇    下一篇

超高效液相色谱-串联质谱法同时测定食用油中痕量的δ-9-四氢大麻酚、大麻酚和大麻二酚

张爱芝, 王全林*, 莫世杰#   

  1. 宁波市产品质量监督检验研究院, 浙江 宁波 315041
  • 收稿日期:2010-07-16 修回日期:2010-08-30 出版日期:2010-11-28 发布日期:2010-11-28
  • 通讯作者: 王全林,博士,教授级高级工程师,从事食品安全检测技术及标准化相关的研究.
  • 基金资助:

    浙江省质量监督检验系统科研计划项目(项目编号: 20080228).

Simultaneous determination of δ-9-tetrahydrocannabinol, cannabidiol and cannabinol in edible oil using ultra performance liquid chromatography-tandem mass spectrometry

  1. Ningbo Academy of Product Quality Supervision & Inspection, Ningbo 315041, China
  • Received:2010-07-16 Revised:2010-08-30 Online:2010-11-28 Published:2010-11-28
  • Contact: Wang QuanLin

PDF

926

摘要： 建立了超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定食用油中δ-9-四氢大麻酚(THC)、大麻酚(CBN)和大麻二酚(CBD)的方法。目标分析物经甲醇提取、中性氧化铝固相萃取柱净化后,采用UPLC-MS/MS分离和检测。实验以氘代四氢大麻酚(THC-D3)为内标物,采用同位素内标法定量。在3个添加水平下,目标物的平均回收率为68.0%～101.6%,相对标准偏差为7.0%～20.1%。方法检出限为0.06～0.17 μg/kg,定量限为0.20～0.52 μg/kg。该方法能够满足食用油中痕量四氢大麻酚、大麻酚和大麻二酚检测的需要。

关键词: δ-9-四氢大麻酚, 超高效液相色谱-串联质谱, 大麻二酚, 大麻酚, 食用油

Abstract: A method for the simultaneous determination of δ-9-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabinol (CBN) in edible oil was developed using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The target compounds were extracted with methanol, purified by an LC-Alumina-N solid phase extraction cartridge, separated and detected by the UPLC-MS/MS. Quantitative analysis was corrected by an isotope internal standard method using δ-9-THC-D3 as internal standard. Average recoveries for the target compounds varied from 68.0% to 101.6% with the relative standard deviations ranging from 7.0% to 20.1% at three spiked levels. The limits of detection (LOD) of the method were from 0.06 ~0.17 μg/kg and the limits of quantification (LOQ) were in the range of 0.20~0.52 μg/kg. The results showed that the method is able to meet the requirements for the simultaneous determination of THC, CBD and CBN in edible oil.

Key words: δ-9-tetrahydrocannabinol, cannabidiol, cannabinol, edible oil, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)